Description
Course section: __________________
REVIEW OF TRANSCRIPTION, RNA-PROCESSING, AND TRANSLATION
Imagine that you are in the lab trying to recreate each of the 3 cellular mechanisms we have
studied during Unit 2 in a test tube.
For each of the 3 processes below, …
• …Fill in list of terms needed (choose terms from the “List of supplies”, below), and
• …Explain the role of each of those terms / why it is required.
• If one item is part of another (e.g., 5’ cap is part of mRNA), list them below one another,
like this:
o “mRNA
▪ 5’ cap.”
1. Transcription of a gene
a.
b.
c. …
2. RNA processing to make a mature mRNA
a.
b.
c. …
3. Translation of an mRNA to make a polypeptide
a.
b.
c. …
List of supplies:
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5’ and 3’ UTRs
5’ cap
A site
Amino acids
Aminoacyl-tRNA synthetase
Anticodons
ATP
Codons
DNA template
E site
Gene
GTP
mRNA
P site
Poly-A tail
Polypeptide
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Pre-mRNA
Promoter
Release factor
Ribonucleotides (A, U, C, G)
Ribosome
RNA polymerase II
rRNA
Spliceosome (is a complex of
RNAs & proteins that removes
introns and joins together exons)
Start codon
Stop codon
TATA box
Transcription factors
tRNAs
[OPTIONAL/ENCOURAGED follow-up practice:
• Draw a model of each mechanism using the items in your list.
• Discuss these mechanisms in Office Hours / appointment / with study group.]
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